作　　者： ; ; ;

机构地区： 河北农业大学中兽医学院

出　　处： 《西北农业学报》 2011年第6期71-74,共4页

摘　　要： 以气相色谱内标法测定变异黄芪中苦马豆素的含量。用超声波协助提取并用溶剂萃取变异黄芪样品,选用D-甘露醇为内标物,采用AT.SE-54毛细管作为色谱柱,柱温210℃,气化室300℃,检测器280℃,分流比30∶1,氢火焰离子化检测器。结果表明,苦马豆素与内标分离良好,在0.015 6-1.0 g/L时线性关系良好（R2=0.999 7）,平均加样回收率为90.6%（RSD=2.41%）。该方法简便、灵敏、易操作,可用于疯草类植物样品中苦马豆素含量的检测。 To develop a reliable method for determination of swainsonine of Astragalus variabilis Bunge by gas chromatography（GC）,swainsonine was extracted with ultrasound-assisted liquid extraction method.AT.SE-54 capillary column was used and temperature was 210℃,injection temperature was 300℃,detector temperature was 280℃,splitting ratio was 30∶1,FID detector,the internal standard was D-Mannito.Swainsonine and D-Mannito were well separated,the standard curve was linear in the concentration range of 0.015 6-1.0 g/L（R2=0.999 7）.The average recovery was 90.6%（RSD=2.41%）.The method can be used to determine swainsonine of locoweed with high sensitivity,precise and good reproducibility.

关 键 词： 苦马豆素 疯草 气相色谱 衍生化

领　　域： [理学] [理学]