作　　者： ; ; ; ;

机构地区： 南方医科大学南方医院

出　　处： 《生物医学工程学杂志》 2011年第4期689-693,697,共6页

摘　　要： 为探索脉冲电刺激下血管内皮细胞与内皮祖细胞(EPC)之间黏附强度的改变,诱导培养外周血EPC,荧光标记后与单层血管内皮细胞共培养,固定电压和频率为5 V和5 Hz,选择1、3、6、9 ms的脉宽对其进行干预,持续刺激24 h后检测贴壁EPC的荧光强度,以荧光比率衡量。结果显示,与对照组相比,3 ms刺激组荧光比率即显著增高,随着脉宽延长,6 ms组达到最大值,但9 ms刺激组却显著下降,提示适宜脉冲电刺激有利于血管内皮细胞与EPC之间的黏附,为电刺激促进血管新生的研究提供新的理论依据。 The present paper is aimed to investigate the effects of pulse electrical stimulation on mutual adhesion of vascular endothelial cell and endothelial progenitor cell（EPC）.EPC was induced from periphery blood,labeled with fluorescence dye and then co-cultured with vascular endothelial cell.With a fixed electric voltage and frequency of 5V and 5Hz,respectively,the co-culture system was continually stimulated for 24h under different pulse width,1,3,6 and 9ms.After pulse stimulation,fluorescence intensity of adherent labeled EPC was measured and converted to fluorescence ratio.Compared to that in the control group, fluorescence ratio of 3ms and 6ms group were significantly larger,while that in the 9ms group was lower.The peak fluorescence ratio value was appeared at 6ms group.It is indicated that suitable pulse electrical stimulation could benefit the adhesion of endothelial cell and EPC.All these results provide a new theoretical basis about why electrical stimulation could contribute to neovascularization.

关 键 词： 脉冲电刺激 血管新生 血管内皮细胞 内皮祖细胞 黏附

领　　域： [生物学] [生物学]