作　　者： ; ; ; ; ; ;

机构地区： 华南理工大学轻工与食品学院

出　　处： 《食品工业科技》 2011年第8期152-156,共5页

摘　　要： 利用酶标仪对铜离子还原能力法Cu-BCS体系(CUPRAC-BCS法)的测定参数进行了优化,确定的检测条件为:CuSO4溶液浓度为1mmol/L、BCS溶液为2mmol/L、体系pH7.4、反应时间20min,490nm检测。应用新建立的"CUPRAC-BCS酶标仪法"对16组凉茶样品抗氧化性进行检测,结果表明该法的测定结果与总酚含量(TPC)、DPPH、Fe3+还原能力法的相关性均达到显著水平(P<0.05)。此方法线性范围为0.1～0.5mmol/LTrolox当量,R2=0.9965,是一种快速、简单、重现性好、具有高通量特点的总抗氧化能力测定方法。 The reaction conditions and analytical parameters of the copper（II）reduction assay with bathocuproinedisulfonic acid disodium salt（BCS）as chelating agent（the CUPRAC-BCS assay）were optimized by the microplate reader.The best factors for analysis at 490nm were as followed：1mmol/L CuSO4,2mmol/L BCS,pH7.4 and reaction time 20min.The antioxidant capacities of 16 herbal tea samples were detected by this novel method.The results showed that CUPRAC-BCS assay was significantly correlated with total phenolic content,DPPH and Fe3＋reducing power assay（P0.05 for all）.The linear range of this method was between 0.1 ~ 0.5mmol/L Trolox equivalents,R2=0.9965.The results showed that CUPRAC-BCS was a fast,simple,suitable and high throughput method for antioxidant capacity assessment.

关 键 词： 铜离子还原能力 抗氧化 凉茶

领　　域： [农业科学] [轻工技术与工程] [轻工技术与工程]