作　　者： ; ; ; ;

机构地区： 华南理工大学轻工与食品学院

出　　处： 《食品工业科技》 2011年第8期84-86,89,共4页

摘　　要： 亲和-超滤载体决定了亲和-超滤膜分离蛋白质的效率。以溶菌酶为对象,探讨了pH、离子浓度、反应时间等操作条件对葡聚糖亲和-超滤载体吸附溶菌酶的影响,随pH增加,亲和-超滤载体对溶菌酶的吸附量呈增长趋势,而随着离子浓度的增加,其吸附作用受到了抑制,此外亲和-超滤载体与溶菌酶的吸附能够在短时间内达到吸附平衡。应用Langmuir方程拟合了等温条件下亲和-超滤载体对溶菌酶的吸附曲线,得到最大理论吸附量为13.65mg/g,解离常数为3.019mg/mL。用醋酸缓冲溶液对载体进行洗脱,测得溶菌酶的平均洗脱率为91.74%。 Affinity-ultrafiltration escort played a critical part in the affinity-membrane filtration process.The adsorption characteristic of the dextran affinty escort to the lysozyme under different pH,ion strength and reaction time were evaluated.The adsorption amount of the lysozyme increased with the increment of pH from 4.2 to 9.2,however,it decreased with the increasing concentration of NaCl.The adsorption equilibrium could be achieved quickly.The adsorption isotherm was analyzed by using the Langmuir model and the maximum adsorption yield was 13.65mg/g,the dissociation constant was 3.019mg/mL.91.74% lysozyme was eluted by acetate buffer,and the recycling usage of the affinity-ultrafiltration escort presented good stability.

关 键 词： 亲和 超滤载体 葡聚糖 吸附特性 溶菌酶

领　　域： [轻工技术与工程] [轻工技术与工程]