作　　者： ; ; ; ; ;

机构地区： 北京大学深圳医院

出　　处： 《中国细胞生物学学报》 2011年第7期777-783,共7页

摘　　要： 本研究采用嵌套缺失和荧光素酶检测技术对鼻咽癌CNE2细胞ezrin基因增强子区进行定位分析。实验结果显示,CNE2细胞中,ezrin基因-1541/-706具有转录激活和转录增强作用,存在转录正调控区和负调控区。对5个潜在转录调控区的进一步研究发现,ezrin基因-1297/-1186对ezrin启动子和SV40启动子具有显著的转录增强作用;其它4个区域对启动子不表现转录调控作用,或表现弱的转录增强作用。结果表明,ezrin基因-1297/-1186是具有增强子作用的关键转录调控区,它有可能与其它潜在转录调控区以共同或协同的方式调控ezrin基因转录。 To localize human ezrin gene enhancer in nasopharyngeal carcinoma cells, a series of reporter gene expression vectors were constructed using nested-deletion method and then were detected via luciferase assay. In CNE2 cells, it was found that ezrin -1541/-706 possessed promoter and enhancer activity, and there were some positive and negative transcriptional regulatory regions within this -1541/-706 region. Further studies on 5 poten- tial transcriptional regulatory regions revealed that ezrin -1297/-1186 enhanced transcription of ezrin promoter and SV40 promoter significantly, other 4 regions showed absent regulation or weaker enhancement on promoters. These data suggested that the ezrin -1297/-1186, a key transcriptional regulatory region as an enhancer, probably regu- lated ezrin transcription in company with other potential regulatory regions.

关 键 词： 鼻咽癌 基因 增强子 转录调控

领　　域： [生物学]