作　　者： ; ; ;

机构地区： 广东海洋大学水产学院

出　　处： 《水产科学》 2011年第7期409-414,共6页

摘　　要： 应用微卫星DNA(SSR)标记技术对福建(FJ)、湛江(ZJ)、北海(BH)地理群体的150尾日本囊对虾个体进行遗传多样性分析。10对微卫星引物在3个群体中共检测到27个等位基因,每个位点的等位基因数为2～4个。平均多态信息含量为0.3074～0.3645,平均观测杂合度为0.2960～0.4212,平均期望杂合度为0.3543～0.4281,表明3个日本囊对虾群体多态性中度偏低,遗传多样性较低。对亚群间近交系数和遗传分化指数计算表明,3个群体多数位点杂合子缺失而纯合子过剩,遗传变异比较弱。平均遗传相似系数为0.9392～0.9625,平均遗传距离为0.0382～0.0627,试验结果表明,3个群体属于同种群体。经UPGMA聚类发现,BH群体与FJ群体亲缘关系较近,而ZJ群体与前两者亲缘关系较远。 The microsatellite（SSR） DNA markers technology was used to analyze the genetic diversities in 150 kuruma shrimp Marsupenaeus japonicus samples found in Fujian province（FJ）,Zhanjiang（ZJ） and Beihai（BH） by 27 alleles in the three groups with 10 SSRs.The allele was found to vary between 2 and 4 for every locus in number,with mean polymorphism information content of 0.3074～0.3645,mean heterozygosity of 0.2960～0.4212,and mean expected heterozygosity of 0.0543～0.4281,indicating that the percentage among the three group shrimps had moderately and low genetic polymorphism.The Fis and Fst values revealed that most heterozygous loci were missed,and the homozygous loci were excess in the three groups,with weak genetic variation.The mean genetic similarity of 0.9392～0.9625 and the mean genetic distance of 0.0382～0.0627 showed that the three groups of shrimp belonged to the same population.Cluster analysis of the three groups by UPGMA confirmed that there was closer linkage between BH and FJ than that in ZJ.

关 键 词： 微卫星 遗传多样性 日本囊对虾

领　　域： [生物学]