作　　者： ; ; ;

机构地区： 武夷学院环境与建筑工程系

出　　处： 《高分子学报》 2011年第7期791-798,共8页

摘　　要： 为了赋予聚丙烯微孔膜(PPMM)选择性吸附低密度脂蛋白(LDL)的能力,发展了一种有效的PPMM表面共价固定肝素的方法.基于紫外引发丙烯酸的接枝聚合,通过碳二亚胺活化,以乙二胺为间隔臂,将肝素共价固定于PPMM表面,获得表面肝素化的PPMM.ATR-FTIR和XPS分析确证了修饰过程中膜表面基团及化学成分的变化.采用静态水接触角测定和血小板黏附实验,考察了肝素化前后膜表面的亲水性及血液相容性,发现在肝素固定化密度为7.39μg/cm2时,膜表面水接触角由(138±7.2)°下降至(35.1±2.7)°,血液相容性也得到明显改善.以酶联免疫吸附测定技术(ELISA)分析了肝素化前后PPMM对单及二元混合蛋白溶液中LDL的吸附与脱吸附性能.结果表明,肝素化PPMM对LDL的吸附量明显增大,且受人血清蛋白(HSA)的干扰较小,说明肝素化PPMM对LDL具有选择性吸附性能;NaCl溶液能将肝素化PPMM表面吸附的LDL洗脱除去,说明肝素化PPMM对LDL的吸附具有可逆性. To endow polypropylene microporous membrane（PPMM） with adsorption selectivity to low-density lipoprotein（LDL）,a versatile method was developed to covalently immobilize heparin on the PPMM surface.This was achieved by grafting acrylic acid（AA） on the PPMM surface by UV irradiation,and subsequent chemical binding of heparin with ethylenediamine as a spacer.The membrane surfaces were characterized by attenuated total reflectance Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy.The hydrophilicity and blood compatibility of the PPMM surface were improved obviously by the surface heparinization.Water contact angle of the membrane surface was decreased from（138±7.2）° for the nascent PPMM to（35.1±2.7）° for the heparinized PPMM with 7.39 μg/cm2 heparin.An enzyme-linked immunosorbent assay（ELISA） was employed to analyze the adsorption of the LDL on the nascent and heparinized PPMMs.It was found that the surface heparinization greatly enhanced the adsorption of LDL both from single protein solution and binary protein solution containing human serum albumin（HSA）,and the heparinized PPMM could selectively adsorb LDL from binary protein solution.In addition,the adsorbed LDL could be desorbed from the heparinized PPMM with sodium chloride solution,which indicated the adsorption was of reversibility.

关 键 词： 聚丙烯微孔膜 表面修饰 肝素化 低密度脂蛋白 吸附

领　　域： [化学工程]