作　　者： ; ; ; ;

机构地区： 郑州牧业工程高等专科学校

出　　处： 《郑州牧业工程高等专科学校学报》 2011年第2期4-6,共3页

摘　　要： 参照GenBank数据库中PCV-2的ORF1基因序列,设计1对特异性引物和1对为反向引物,利用常规PCR扩增ORF1基因片段,再利用反向PCR扩增ORF1基因片段侧翼序列,将2次PCR产物克隆测序,经BLAST分析确认为PCV-2基因组序列,利用Vector NTI 8.0软件将两个序列进行拼接获得PCV-2全基因组。结果表明,克隆到的PCV-2全基因组长为1767bp。 According to the ORF1 gene sequence of PCV-2 from GenBank,we designed a pair of primers for conventional PCR and a pair of primers for reverse PCR.The fragment of ORF1 amplified by conventional PCR and the flank fragment amplified by reverse PCR were cloned and sequenced.Sequences of the both PCR products were confirmed to be the sequence of PCV-2 genome by BLAST.Then,the both fragment sequences were spliced to be the complete genome of the PCV-2.The results showed that these genomes were all 1767bp in size.

关 键 词： 猪圆环病毒 型 基因组 反向

领　　域： [农业科学] [农业科学] [农业科学]