作　　者： ; ; ; ; ;

机构地区： 西北师范大学化学化工学院生态环境相关高分子材料教育部重点实验室

出　　处： 《科学通报》 2011年第17期1360-1366,共7页

摘　　要： 将难溶性的锌卟啉(ZnTpHPP)与牛血清白蛋白(BSA)结合,制得一类新型水溶性生物高分子金属卟啉配合物(BSA-ZnTpHPP).通过紫外可见光谱(UV-Vis)、圆二色谱(CD)及非变性聚丙烯酰胺凝胶电泳(Native-PAGE)对BSA-ZnTpHPP的结构进行了表征,发现二者以配位键结合,BSA与锌卟啉以较低比例结合时蛋白质二级结构保持.考察了BSA-ZnTpHPP的光敏感性,发现BSA-ZnTpHPP在光照条件下易变成三重激发态,可以将电子转移给甲基紫精(MV2+).以三乙醇胺(TEOA)为电子供体,甲基紫精(MV2+)为电子中继体,以BSA-ZnTpHPP/MV2+/TEOA/胶体Pt四组分体系考察了BSA-ZnTpHPP的光诱导水解产氢性能,结果表明,这类水溶性生物高分子金属卟啉光敏剂具有良好的光解水产氢性能. A novel water soluble biopolymer metalloporphyrin complex（BSA-ZnTpHPP） was prepared by combination of insoluble meso-tetra（4-hydroxyphenyl） porphyrin zinc complex（ZnTpHPP） and bovine serum albumin（BSA）.The complex was characterized by UV-Vis spectroscopy,circular dichroism,and native polyacrylamide gel electrophoresis.The secondary structure of BSA was maintained after coordination of the metalloporphyrin complex at a low ratio.The photosensitivity of BSA-ZnTpHPP was also investigated.Its fluorescence was quenched by methyl viologen（MV2＋）,which suggests that photo-induced electron transfer occurs from BSA-ZnTpHPP to MV2＋ in the BSA-ZnTpHPP/MV2＋/triethanolamine（TEOA） system.Under light,BSA-ZnTpHPP was easily excited to the triplet state,and then transferred an electron to MV2＋.The BSA-ZnTpHPP/MV2＋/TEOA/colloidal Pt system was applied to photosensitized reduction of water for preparation of hydrogen.The water-soluble biopolymer metalloporphyrin complex was an excellent biopolymer photosensitizer.

关 键 词： 生物高分子 光敏剂 金属卟啉 白蛋白 电子转移反应 产氢

领　　域： [理学] [理学]