作　　者： ; ; ; ;

机构地区： 河南农业大学农学院

出　　处： 《玉米科学》 2011年第3期43-46,共4页

摘　　要： 通过RT-PCR方法和基因克隆技术对黄早四Rbx1基因进行分子克隆和序列分析,构建黄早四Rbx1的原核表达系统,成功表达出带有His标签的黄早四Rbx1融合蛋白,并进一步利用His抗体,用蛋白免疫印迹的方法对重组蛋白进行鉴定。结果表明,重组蛋白能够与His抗体发生免疫反应,验证了黄早四Rbx1在原核表达系统中成功表达。 Molecular cloning and sequence analysis of Huangzaosi Rbxl were carried out based on RT-PCR method and gene cloning technique. At the same time, the prokaryotic expression system of Huangzaosi Rbxl was constructed and the recombinant protein with a His-tag was successfully expressed. Furthermore, western blot with His antibody was used to identify the recombinant protein. The results showed that the recombinant protein had immune reaction with His antibody, and verified that Rbxl from Huangzaosi could be successfully expressed in prokaryotic expression system.

关 键 词： 玉米 黄早四 泛素连接酶 分子克隆 原核表达 免疫印迹

领　　域： [农业科学]