作　　者： ; ; ; ; ; ; ;

机构地区： 中国水产科学研究院珠江水产研究所

出　　处： 《水产学报》 2011年第5期719-728,共10页

摘　　要： 采用生化分析方法研究了盐度变化(24、14、4及对照34)对斜带石斑鱼幼鱼[体质量(19.59±0.25)g]相关生理生化因子的影响,不同盐度处理后1、3、6和9 d检测鳃丝Na+/K+-ATP酶、血清中血糖、天门冬氨酸氨基转移酶(AST)、溶菌酶,肝脏超氧化物歧化酶(SOD)含量及H.E染色观察鳃丝氯细胞的变化。结果显示,各盐度试验组斜带石斑鱼幼鱼的Na+/K+-ATP酶活性变化基本一致,均在第3天达到最高值,随后下降,至第6天达到稳定。鳃丝氯细胞数量在盐度24试验组中变化不大,在盐度14试验组中则略有减少,在盐度4试验组中鳃丝氯细胞6 d后出现变化,氯细胞数量减少,胞核较大,H.E染色较深,细胞处于较原始的状态。各盐度试验组斜带石斑鱼幼鱼的血糖水平变化趋势相一致,均在第6天到达峰值,随后呈下降趋势。AST水平在盐度24、盐度4试验组随时间的延长呈上升趋势,在第9天后到达各自的最高值,盐度14试验组则呈现持续下降趋势。SOD活性随时间的延长呈现先降低后上升的趋势,在第3天时各试验组达最小值,但试验组间SOD活性差异显著(P<0.05)。溶菌酶活性随时间的延长呈上升趋势,试验组在第6天达到的峰值,随后下降并趋于稳定,试验组间差异显著(P<0.05)。结果表明,斜带石斑鱼幼鱼通过盐度驯养,由盐度34水体逐渐淡化至盐度为24、14、4的水体中,能较快适应盐度低至4的水环境。 The objectives of this work were to study the effects of salinity change on the physiological chemical factors of Epinephelus coioides juvenile[body weight：（19.59±0.25）g]by a biochemical analysis.The gill Na＋/K＋-ATPase,plasma glucose,aspartate carbamyl transferase（AST）,the activity of SOD and lysozyme of Epinephelus coioides juvenile were assayed after being treated with salinity[（4,14,24 and 34 as contro1）]in 1,3,6 and 9 d At the same time,the gill chloride cells were observed using an H-E staining.The results showed that Na＋/K＋-ATPase activity in all groups showed similar tendency and the concentration reached the maximum level on 3rd day,then decreased and keep it constant on 6th day.The number of gill chloride cells did not show significant difference in salinity 24,but decreased slightly in salinity 14 during the experiment period.The changes in number,nucleus size and color of gill chloride cells in salinity 4 were observed on 6th day.The serum glucose level in all groups showed similar tendency and the concentration reached the maximum level on 6th day,then decreased.The serum AST level showed similar tendency both in salinity 24 and in salinity 4 and the concentration reached the maximum level on 9th day.However,the serum AST level continuously decreased in salinity 14 during the experiment period.The hepatic SOD activity in all test groups showed a tendency of decreasing at the beginning and increasing later,and reached the minimum level on 3rd day.There were significant differences in the hepatic SOD activity among all test groups（P0.05）.The serum lysozyme activity in all test groups showed a tendency of increasing at the beginning and reached the maximum level on 6th day,then decreased and gradually stable.There were significant differences in serum lysozyme activity among all test groups（P0.05）.When the salinity gradually decreased from 34 to 24,14 and 4,these physiological chemical indicators showed similar tendency in all test groups,suggesting that E.coioides is typic

关 键 词： 斜带石斑鱼 盐度胁迫 酶 氯细胞 血糖 天门冬氨酸氨基转移酶 超氧化物歧化酶 溶菌酶

领　　域： [生物学] [生物学] [农业科学]