机构地区: 暨南大学理工学院食品科学与工程系
出 处: 《植物检疫》 2011年第3期35-38,共4页
摘 要: 以油菜内源基因PEP、抗除草剂基因(BAR、PAT)、筛选基因NPTII、常见启动子(CaMV35S、FMV35S)和终止子NOS为检测对象,通过研究不同引物终浓度的配比以及退火温度对转基因菜籽粕多重PCR检测的影响,建立了菜籽粕转基因成分7重PCR检测体系。结果表明,本研究所建立的检测体系能有效检测出菜籽粕以及其他作物(大豆、玉米、大米、棉花籽)中的转基因成分,检测过程简便、准确,值得推广应用。 Muhiplex PCR detection system for rapeseed meal is established with targeted genes including FMV35S, CaMV35S promoter, NOS terminator, NPTII, PAT, BAR, and an endogenous gene PEP. The PCR conditions for the multiplex PCR analysis were optimized according to the primer concentration and annealing tem- perature. Results showed that this multiplex PCR system can detect the genetically modified components from rapeseed meal and other crops( soybean, corn, rice, cotton seeds) , with simple, accurate and highly specific advantages.
领 域: [生物学]