作　　者： ; ; ; ;

机构地区： 赣南医学院药学院

出　　处： 《光谱实验室》 2011年第3期1089-1092,共4页

摘　　要： 分别采用常规回流提取法、超声提取法、微波辅助萃取法及索氏提取法对中药山豆根进行提取;以芦丁为对照品,采用分光光度法测定提取物中总黄酮的含量;以总黄酮的含量为考察指标,优选出最佳提取方法。结果表明,4种提取方法中,常规回流提取法总黄酮含量最高,微波辅助萃取法和超声提取法总黄酮含量较高,索氏提取法总黄酮含量最低;综合考虑,认为微波辅助萃取法和超声提取法具有高效、省时等优点,提取效果较好。 The contents of total flavonoids were extracted from Sophora tonkinensis Gapnep.by different methods of regurgitant extracting,Soxhlet extracting,microwave aided extracting and ultrasonic wave extraction,that were determined by spectrophotometry with the rutin as reference substance.The total flavonoids as reference,the best conditions were optimized.The results showed that the contents of total flavonoids in four extraction methods were highest in the samples extracted by regurgitant extracting,and microwave aided extracting and ultrasonic wave extraction were second higher,then Soxlet extracting were lowest.The microwave aided extracting and the ultrasonic wave extraction were suitable for extracting total flavonoids from Sophora tonkinensis Gapnep.with low extraction time and high efficiency.

关 键 词： 山豆根 总黄酮 提取方法 分光光度法

领　　域： [理学] [理学]