作　　者： ; ; ; ; ; ;

机构地区： 青岛农业大学

出　　处： 《动物医学进展》 2011年第4期76-79,共4页

摘　　要： 对不同地区猪源大肠埃希菌磺胺药物耐药性进行系统性调查研究。对从山东、内蒙古、山西分离到的161株猪源大肠埃希菌测定磺胺药物对其最低抑菌浓度(MIC值),结果表明大肠埃希菌对磺胺药物高度耐药,对磺胺异恶唑(SF)和新诺明(SMZ)的耐药率分别为91.30%和90.06%。同时根据GenBank上已有的二氢叶酸合成酶基因Sul1、Sul2、Sul3的基因序列,设计3对引物,对耐药菌株的磺胺药耐药基因Sul1、Sul2、Sul3进行检测,药敏试验结果与耐药基因检测结果在不同地区菌株中的符合率高达80%以上。 The systematic investigation and study on sulfonamide resistance in Escherichia coli isolated from pigs in different areas were conducted.The minimum inhibitory concentrations（MIC）of sulfonamides were detected in 161 Escherichia coli isolates from pigs in Shandong,Inner Mongolia and Shanxi.The result showed that the sulfonamide resistance rates of the isolates were very high,the resistance rate to sulfafurazole（SF）was 91.30% and to sulfamethoxazole（SMZ）was 90.06%.Three pairs of primers were designed according to the gene orders of Dihydrofolate synthetase sul1,sul2 and sul3 available in GenBank.Sulfonamides resistance genes sul1,sul2 and sul3 were detected.The consistency rate between results of the drug sensitivity test and that of the resistance gene test was above 80% in Escherichia coli isolates from three provinces.

关 键 词： 猪 大肠埃希菌 磺胺类药物 药敏试验 耐药基因

领　　域： [农业科学] [农业科学] [农业科学]