作　　者： ; ; ; ;

机构地区： 广州军区广州总医院

出　　处： 《生物技术通讯》 2011年第2期203-207,共5页

摘　　要： 目的:筛选慢性髓细胞性白血病(CML)病人骨髓单个核细胞与正常人的差异表达基因,探讨CML的发病机制。方法:提取正常人和CML病人单个核细胞的RNA,逆转录成cDNA并用地高辛标记,应用全基因组表达谱基因芯片对差异表达基因进行研究,采用Jubilant病理/疾病分类法对CML相关差异表达基因进行分析。结果:共筛选出CML相关差异表达基因43个,其中13个基因表达上调,30个基因表达下调,上调基因较多地参与信号转导、蛋白代谢、修饰、核酸代谢、mRNA转录调控等生物学过程;下调的基因主要涉及核酸代谢、信号转导、细胞及个体发育、细胞周期、免疫及防疫等方面。结论:CML病人与正常人的单个核细胞中有43个基因具有显著的表达差异,筛选获得的差异靶基因在CML恶性转化过程中发挥了重要作用,这为药物靶基因的筛选提供了理论基础。 Objective：To screen differential expressed genes in marrow mononuclearcell of chronic myelogenous leukemia（CML） patient in order to develop the mechanism of CML.Methods：RNA were extracted in marrow mononuclearcell of both normal person and CML patient marked with digoxin.Differential expressed genes were analyzed by ABI 1700 bioarray and sorted by Jubilant physiology/disease categories.Results：A total of 6706 genes had significant differential expressed 43 genes relating to CML which have 13 genes up-regulated relating to signal transduction,protein metabolism and modification,nucleoside,nucleotide and nucleic acid metabolism,mRNA transcription,et al;while 30 genes down-regulated relating to nucleoside,nucleotide and nucleic acid metabolism,protein metabolism and modification,signal transduction,developmental processes,cell cycle,et al.Conclusion：43 genes relating to CML have significant differential expressed,and play an important role in the process of CML malignant transformation.

关 键 词： 慢性髓细胞性白血病 寡核苷酸微阵列 基因表达谱

领　　域： [生物学] [生物学]