作　　者： ; ; ; ; ;

机构地区： 华南农业大学食品学院

出　　处： 《食品工业科技》 2011年第5期209-211,共3页

摘　　要： 以海藻酸钠、壳聚糖为材料共固定化菠萝茎蛋白酶,并对固定化条件以及固定化酶的酶学特性进行了研究。通过单因素及正交实验确定固定化酶的最佳条件为:酶用量0.4mg/g、海藻酸钠浓度3.0%、壳聚糖浓度1.0%、CaCl2浓度5.5%、固化时间90min。固定化后的酶重复使用4次后,相对酶活力仍保留80%以上,固化效果较好。同时,固定化酶最适反应温度和热稳定性提高,反应pH向碱性偏移,酶学特性得到了改善。 Sodium alginate and chitosan materials were fixed to the stem bromelain,and the immobilization conditions and characteristics of immobilized stem bromelain were studied.Through single factor and orthogonal experiment,the best conditions for immobilized stem bromelain were determined as follow：enzyme dosage of 0.4mg/g,3.0% sodium alginate concentration,chitosan concentration of 1.0%,CaCl2 concentration of 5.5%,immobilized time of 90min.The relative activity of immobilized stem bromelain after repeated use four times remained above 80%.Meanwhile,the optimal pH shifted to alkalescence,the immobilized enzyme optimal temperature,thermal stability and enzymatic characteristics had been improved.

关 键 词： 海藻酸钠 壳聚糖 菠萝茎蛋白酶 固定化

领　　域： [轻工技术与工程] [轻工技术与工程]