作　　者： ; ; ; ; ; ;

机构地区： 中国科学院水生生物研究所

出　　处： 《水生生物学报》 2011年第2期256-261,共6页

摘　　要： 实验比较了不同pH胁迫对异育银鲫(Carassius auratus gibelio)皮质醇激素和非特异性免疫的影响。3个实验组水质的pH分别为6.0、8.5、9.5,对照组是pH为7.4的曝气自来水。分别在实验的第0、14和30天采血,血浆用于测定皮质醇含量和溶菌酶活性;分离的白细胞,采用流式细胞术测定吞噬活性和呼吸暴发。在实验的第4天多取1次血样,用于皮质醇含量测定。在实验的第31天,测量鱼的体重,并用柱状黄杆菌(Flavobacterium columnare)浸泡攻毒,7d后统计死亡情况。结果显示:在pH 6.0的胁迫下,白细胞的吞噬活性在第14、30天显著降低,而呼吸暴发和皮质醇含量没有显著变化,溶菌酶活性和生长与对照组没有显著差异,用黄杆菌攻毒也没有出现死亡。而在pH 8.5和9.5的胁迫下,皮质醇含量在第14、30天显著升高,白细胞的吞噬活性、呼吸暴发显著降低;在pH 9.5胁迫下的溶菌酶活性显著降低,用柱状黄杆菌攻毒两组都出现死亡。实验表明pH为8.5和9.5的胁迫严重影响了异育银鲫的非特异性免疫功能,流式细胞术能较客观测定白细胞的吞噬活性和呼吸暴发,可用于评价胁迫对鱼类健康的影响。 Effects of pH stress on cortisol and non-specific immunity of Carassius auratus gibelio were investigated.Acidity and Alkalinity of the aquaculture water were maintained at pH 6.0,7.4,8.5 and 9.5,where pH 7.4 was used as control.pH value of the aquaculture water was adjusted by HCl（1 N） or NaOH（1 N）.Fish blood was sampled at 0,14 and 30 days during the experiment.Leucocytes isolated by 55% percoll were used for determination of phagocytosis and respiratory burst activity by flow cytometry,and the plasma was used to survey the cortisol concentration and lysozyme activity.Fish weight was measured when pH stress protocol finished.The fish was then challenged by the bacteria Fla-vobacterium columnare,and dead fish was recorded after 7 days of the challenge.Under the stress of pH 6.0,phagocy-tosis of leucocytes decreased significantly at the 14 and 30 day,but no significant differences were found in respiratory burst activity and cortisol level.There were significant differences in lysozyme activity and growth between pH 6.0 and control group.No fish died from the bacteria challenge.Under pH 8.5 and 9.5 stress,the cortisol concentration increased significantly,and phagocytosis and respiratory burst activity decreased significantly at the 14 and 30 day.The challenge of bacteria resulted in fish death.The results suggested that non-specific immunity and growth of the carp was heavily affected by the pH 8.5 and 9.5 stress,and phagocytosis and respiratory burst activity of fish leucocytes determined by flow cytometry could assess the influence of stress on fish health.

关 键 词： 胁迫 异育银鲫 皮质醇 溶菌酶活性 吞噬活性 呼吸暴发 流式细胞术

领　　域： [生物学] [生物学]