作　　者： ; ;

机构地区： 中山大学生命科学学院

出　　处： 《中山大学学报（自然科学版）》 1999年第5期82-86,共5页

摘　　要： 用重量法和柱层析法从苦茶Ｃａｍｅｌｌｉａ ａｓｓａｍｉｃａ ｖａｒｋｕｃｈａ Ｃｈａｎｇ ｅｔ Ｗａｎｇ 的嫩梢中分离出３ 种嘌呤生物碱， 经薄层层析、熔点测定、元素分析、核磁共振和质谱等对其结构的测定，鉴定为咖啡碱、１ ， ３ ， ７ ， ９四甲基尿酸和可可碱， 其百分含量分别为０８８％ ， ０８４ ％ 和０２９ ％ ． 样品水提物经氯仿萃取后直接由高压液相色谱分析， 测得苦茶含咖啡碱１９３２ ９％ ，四甲基尿酸１２９４ １ ％ ， 可可碱０５８５ ５ ％ ， 茶叶碱００１２ ８％ ． Purine alkaloids in Camellia assamica var. Kucha Chang et Wang were investigated by extraction combined with silica gel column chromatography. Three products were isolated. Product A and C were determined as caffeine and theobromine by TLC and melting point compared with standards. The structure of product B were determined by MS and 1HNMR, the element analysis and melting point for 1,3,7,9 tetramethyluric acid.The purine alkaoids were consisted of caffeine, 1,3,7,9 tetramethyluric acid, theobromine and theophylline in the leaves of C.assamica var. kucha , and their content was 1 932 9%, 1 294 1%, 0 585 5% and 0 012 8% respectively by HPLC.The distribution of new type of purine alkaloids and such high content of tetramethyluric acid in wild tea tree resources is firstly reported.

关 键 词： 苦茶 嘌呤生物碱 咖啡碱 山茶属

领　　域： [生物学]