作　　者： ; ; ; ; ;

机构地区： 华南农业大学资源环境学院农药与化学生物学教育部重点实验室

出　　处： 《华南农业大学学报》 2011年第2期44-47,共4页

摘　　要： 建立了同时检测水果中9种农药（1-六六六、3-六六六、甲基毒死蜱、毒死蜱、苯氟磺胺、灭菌丹、p,p′-DDD、甲氰菊酯、三氟氯氰菊酯）残留的分散固相萃取-气相色谱联用方法.水果样品中残留农药采用乙腈提取,使用N-丙基乙二胺键合固相吸附材料（PSA）和C18吸附剂分散固相萃取净化,采用DB-1701毛细管柱分离,电子捕获检测器（ECD）检测,外标法定量.在设定的色谱条件下,9种农药在0.000 5-0.1000 mg/L范围内线性良好,检出限0.000 2-0.002 0 mg/kg;添加质量浓度为0.01-0.10 mg/kg时,9种农药平均回收率为74.4%-115.9%,相对标准偏差为2.3%-13.7%.该方法操作简单快速、准确度高,能够满足水果中多种农药残留限量标准的检测要求. A rapid,simple and sensitive method was developed for the simultaneous determining of nine pesticide residues（1-BCH,3-BCH,chlorpyrifos,chlorpyrifos-methyl,dichlofluanid,folpet,p,p′-DDD,fenpropathrin,cyhalothrin） in fruits by dispersive solid phase extraction-gas chromatography.The target pesticides in fruits were extracted by acetonitrile and cleaned up by dispersive solid phase extraction by using primary secondary amine（PSA） and C_18 dispersive sorbent.The analytes were detected by gas chromatography with electron capture detector（ECD）,and quantified by external standard.With the optimal chromatogram conditions,the good linearities were obtained ranging from 0.000 5 mg/L to 0.100 0 mg/L for nine pesticides.The detection limits of nine pesticides were from 0.000 2 mg/kg to 0.002 0 mg/kg.The average recoveries ranged from 74.4% to 115.9% at fortified levels from 0.01mg/kg to 0.10 mg/kg.The relative standard deviations（RSD） were less than 13.7%.The method of dispersive SPE-GC established in this study could be used for detecting the pesticide residues in fruits.

关 键 词： 分散固相萃取 气相色谱法 农药残留 水果

领　　域： [农业科学] [农业科学] [农业科学]