作　　者： ; ; ;

机构地区： 运城学院应用化学系

出　　处： 《理化检验（化学分册）》 2011年第3期266-268,共3页

摘　　要： 在一片聚甲基丙烯酸甲酯(PMMA,50mm×40mm×3mm)上用激光雕刻机雕刻一条宽200μm,深100μm,长9 cm的蜿蜒形流通池,其上盖以另一片大小相同的PMMA并在1.6MPa压力和108℃温度条件下使之结合成一体,从而制成了微流动注射芯片。基于氢化可的松对鲁米诺-铁氰化钾化学发光体系的增敏作用,提出了微流动注射芯片-化学发光法测定氢化可的松含量的方法。当化学发光体系中鲁米诺的浓度为8.0×10^(-5)mol·L^(-1)、铁氰化钾浓度为4.0×10^(-5)mol·L^(-1)、体系的pH值为12时,氢化可的松发光强度的增加值与其质量浓度在8.0×10^(-5)～8.0×10^(-2)g·L^(-1)范围内呈线性关系,检出限(3S/N)为4.0×10^(-5)g·L^(-1)。方法应用于食品中氢化可的松残留量的测定,回收率在96.5%～104.0之间。 A sinuous flow-cell with 200 9m in width, 100 9m in depth and 9 cm in length was carved with laser carving machine on a piece a poly methyl mathacrylate （PMMA, 50 mm×40mm×3mm） which was covered and bonded with another piece of PMMA of same size under 1.6 MPa and 108 ℃ to form the micro-flow injection chip. Based on the sensitivation of chemiluminescence （CL） of the reaction system of luminol-K3Fe（CN）6 by hydrocortisone, and by using the micro FI-chip, a method of FI-CL determination of hydrocortisone was proposed. The optimum concentrations of luminol and K3Fe（CN）6 were found to be 8. 0×10^-5 and 4. 0 ×10^-5mol·L^-1 respectively, and pH value of the system was found to be 12. Linear relationship between the magnitude of increase in CL intensity and mass concentration of hydrocortisone was obtained in the range of 8. 0 ×10^-5 - 8. 0 ×10^-2g·L^-1 with detection limit （3S/N） of 4. 0 ×10^-5g·L^-1. The proposed method was used in the analysis of food sample, giving values of recovery in the range of 96.5%-104. 0%.

关 键 词： 化学发光 微流动注射芯片 氢化可的松

领　　域： [理学] [理学]