作　　者： ; ; ; ; ;

机构地区： 华南农业大学园艺学院

出　　处： 《中国农学通报》 2011年第8期150-155,共6页

摘　　要： 调查了44份芥蓝种质的植物学性状,并利用RAPD分子标记分析了其遗传多样性。结果从150条随机引物中筛选出20个引物,20个引物共扩增出177条谱带,其中多态谱带105条,平均每个引物扩增出8.9条谱带和5.3条多态性谱,多态性比率为59.32%。基于RAPD标记,利用NTSYS-pc2.11构建了聚类树状图谱,遗传距离为0.70时,44份芥蓝资源可聚成6大类群。芥蓝种质存在着一定的遗传多样性,但原产华南而且主要产区也在华南,遗传多样性要小于芸薹属其他蔬菜。 The morphological characteristics of 44 Chinese kale accessions were investigated and the DNA polymorphism was analyzed by randomly amplified polymorphic DNA （RAPD） marker.20 primers selected from 150 primers amplified a total of 177 bands,of which 105 were polymorphic,with an average of 8.9 bands and 5.3 polymorphic fragments per primer.The polymorphic percentage was 59.32%.The dendrogram based on RAPD data was constructed by NTSYS-pc2.11.Cluster analysis showed that 44 Chinese kale accessions could be divided into six groups at the level of D=0.70.Because Chinese kale was from south China and the main area was in south China,the genetic diversity of Chinese kale was smaller than otherBrassica vegetables.

关 键 词： 芥蓝 种质资源 遗传多样性

领　　域： [农业科学] [农业科学]