作　　者： ; ; ; ; ;

机构地区： 湛江师范学院生命科学与技术学院

出　　处： 《植物生理学报》 2011年第2期141-146,共6页

摘　　要： 启动子是调控基因转录的一段DNA,也是构建基因工程表达载体的重要元件。天然启动子在表达强度和特异性等方面存在一定的局限性。采用人工构建的方法,有望得到诱导因子广、本底活性低、表达强度高、启动表达快等特点的启动子。本文综述了人工启动子在诱导表达、组织特异性表达、高效表达等方面的研究进展。 Promoter is a piece of DNA fragment that regulates gene transcription by allowing RNA polymerase and transcription factors binding to it.Promoter is also an important element in the expression vectors in genetic engineering.However,there are some limitations in natural promoter,such as low expression activity and low specificity.Alternatively,artificial constructed promoters are expected to overcome those limits and achieve advantages,such as more induction factors,low basal activity,high expression activity and quick expression initiation.In this paper,we reviewed research advances in artificial promoter constructions in terms of the inducible expression,tissue-specific expression and high-efficiency expression.

关 键 词： 人工启动子 诱导表达 组织特异性表达 高效表达

领　　域： [生物学]