作　　者： ; ; ; ; ; ;

机构地区： 广东省农业科学院

出　　处： 《安徽农业科学》 2011年第5期2807-2809,2812,共4页

摘　　要： [目的]对产几丁质酶菌株发酵产酶活性工艺进行优化。[方法]将筛选得到的1株产几丁质酶菌株G-254进行驯化培养,通过单因素试验考察了不同碳源、氮源和无机盐对菌株产酶活的影响;进行响应面试验,以菌株发酵所产酶活为响应值,确定最佳的发酵产酶工艺条件。[结果]菌株G-254发酵产几丁质酶最佳发酵条件为,葡萄糖8%,牛肉膏料5%,硫酸镁0.07%,在此条件下获得的几丁质酶活为6.86 U。[结论]提高了菌株发酵产几丁质酶酶活,为后续工业化发酵生产奠定了基础。 [Objective] The aim of this study was to optimize the conditions of chitinase-produce strains.[Method] A kind of screened chitinase-produce strain G-254 was habituated cultured,and then the single factor experiment was carried out to explore the effects of different carbon source,nitrogen source and inorganic salt on the activity of produced chitinase;the response surface test was used to determine the optimal conditions for chitinase production.[Result] The optimal conditions for chitinase production were： 8% of glucose,5% of beef extract and 0.07% of MgSO4,and the activity of chitinase reached the maximal value（6.86 U） under these conditions.[Conclusion] The study improved the activity of chitinase produced by strain G-254 and provided good foundation for industrial production.

关 键 词： 蚕蛹壳 几丁质 降解 酶活 优化

领　　域： [农业科学] [农业科学] [生物学]