作　　者： ; ; ; ; ; ; ;

机构地区： 广东省农业科学院

出　　处： 《果树学报》 2011年第2期215-220,共6页

摘　　要： 为了从DNA分子水平上探讨广东境内果梅种质资源遗传多样性状况,利用荧光标记AFLP技术,对采自广东境内的57份果梅种质进行研究,筛选出适于果梅AFLP分析的8对MseⅠ和EcoRⅠ引物组合,这些引物可以将供试材料完全分开。利用8对引物组合对57份果梅种质进行总基因组DNA水平上的多态性检测,得到了清晰的多态性指纹图谱,共扩增出33 117个位点,其中多态性位点9 624个,多态率为29.06%。UPGMA聚类表明,各品种间相似系数为0.56～0.90,多态性比例介于0.172 0～0.423 9,说明在分子水平上果梅品种的遗传多样性并非形态学性状体现的那样丰富,依相似系数0.75水平,可以将57份果梅种质资源分成8组。 In order to study the genetic diversity of Prunus mume germplasm in Guangdong on molecular level,57 Prunus mume germplasm were analysed by using AFLP technique.Eight AFLP primer combinations（MseⅠand EcoRⅠ） were screened out from 64 pairs of primers.The clear fingerprint maps were obtained from the 57 germplasm.The results suggested that the eight pairs of primers could be used to distinguish all the materials tested.A total of 33117 fragments were generated from the eight pairs of AFLP primers,9 624 of which were polymorphic.The level of mean polymorphism was 29.06%.The genetic similarity indexes of 57 Prunus mume germplasm ranged from 0.56 to 0.90,and the ratio of polymorphic varied from 0.172 0 to 0.423 9.The results of genetic diversity analysis among 57 Prunus mume germplasm indicated that the diversity was more abundant on morphological level than on molecular level.The 57 Prunus mume germplasm were clustered into 8 groups by UPGMA dendrogram at genetic similarity coefficient value of 0.75.

关 键 词： 果梅 种质 遗传多样性

领　　域： [农业科学] [农业科学]