作　　者： ; ; ; ;

机构地区： 徐州工程学院食品生物工程学院

出　　处： 《食品科学》 2011年第6期7-10,共4页

摘　　要： 以木瓜蛋白酶和AS1398中性蛋白酶组成的复合酶对猪血红蛋白进行水解,制备富血红素多肽,考察底物质量分数、加酶比例、水解pH值和水解温度等因素对水解率、蛋白质回收率、血红素含量等指标的影响。结果表明:底物质量分数是影响水解率和蛋白质回收率指标的最重要因素;pH值是影响血红素含量指标的最重要因素;复合酶水解猪血红蛋白制备富血红素多肽的最佳工艺条件为底物质量分数6%、pH8、水解温度55℃、加酶比例5:5(木瓜蛋白酶:中性蛋白酶)。在此工艺条件下,猪血红蛋白水解产物具有较高的蛋白质回收率和血红素含量。 In order to prepare a heme-rich polypeptide product,mixed papain and neutral protease were used for the hydrolysis of porcine hemoglobin.The effects of substrate concentration,mixing ratio for papain and neutral protease,hydrolysis pH and hydrolysis temperature on hydrolysis degree,protein recovery rate and heme content were investigated.The results indicated that substrate concentration was the most important factor that affects hydrolysis degree and protein recovery rate.pH was the most important factor that affects heme content.The optimal hydrolysis conditions for the preparation of heme-rich polypeptide products were substrate concentration of 6%,pH 8,hydrolysis temperature of 55 ℃ and papain-to-neutral protease ratio of 5：5.Under the optimal preparation conditions,relatively high protein recovery rate and heme content for the hydrolysis of porcine hemoglobin were achieved.

关 键 词： 猪血红蛋白 血红素 多肽 复合酶 水解

领　　域： [轻工技术与工程] [轻工技术与工程]