作　　者： ; ; ; ; ; ; ; ;

机构地区： 江苏大学基础医学与医学技术学院

出　　处： 《食品科学》 2011年第5期244-248,共5页

摘　　要： 目的:观察蜂胶和铁补充对运动大鼠血液铁状态指标和血浆自由基代谢的影响,研究蜂胶对运动和/或铁补充过程中产生的氧化损伤的抗氧化保护作用。方法:40只3月龄Sprague-Dawley雄性大鼠,被随机分为5组:对照组(C组)、运动组(E组)、运动补铁组(EI组)、运动补蜂胶组(EP组)和运动补铁同时补蜂胶组(EIP组),每组8只。6周递增负荷跑台训练后检测各组大鼠红细胞指标(红细胞数(RBC)、血红蛋白(Hb)、红细胞比容(Hct))和血浆铁状态指标(血浆铁(PI)和总铁结合力(TIBC));并检测血浆MDA和SOD值。结果:E组红细胞和铁状态指标显著低于C组,MDA值显著高于C组,SOD值显著低于C组。与E组比较,EI组、EP组和EIP组的红细胞、铁状态指标均显著升高;EI组MDA值显著高于E组,SOD值显著低于E组;EP组和EIP组MDA值显著低于E组,SOD值显著高于E组。EIP组MDA值显著低于EI组,SOD值显著高于EI组,但与EP组无显著差异。结论:虽然铁补充能改善运动大鼠贫血和低铁状态,但增加了大鼠氧化应激风险。蜂胶能有效提高机体清除自由基能力,减少脂质过氧化,增强抗氧化酶活性;对改善运动大鼠贫血和低铁状态也有一定作用。 Objective： To explore the effects of propolis and iron supplements on iron status and free radical content in blood of exercise rats,and to investigate the antioxidative protection of propolis on oxidative damage derived from exercise and/or iron supplement.Methods： Forty Sprague-Dawley male rats aged 3 months were randomly divided into 5 groups of 8 each： control group（C group）,exercise group（E group）,exercise plus iron supplement group（EI group）,exercise plus propolis supplement group（EP group） and exercise plus supplement with iron and propolis group（EIP group）.The rats were subjected to treadmill training with an incremental loading for 6 weeks.Red blood cell indices including red blood cell count（RBC） and hemoglobin（Hb） and hematocrit（Hct）,plasma iron status including plasma iron（PI） and total iron binding capacity,and serum MDA and SOD contents of rats were measured.Results： Compared with C group,a significantly lower level of red blood cell indices,iron status and SOD,and an obvious higher level of MDA were observed in E group.Compared with E group,a significantly higher level of red blood cell indices and iron status was observed in rats from EI,EP and EIP groups;meanwhile,MDA content in rats from EI group was higher than that in E group;SOD content in rats from EI group was lower than that in E group.MDA content of rats in EP and EIP groups was lower than that in E group and SOD content of rats in EP and EIP groups was higher than that in E group.MDA content in rats from EIP group was lower than that in EI group and SOD content in rats from EIP group were higher than that in EI group.No significant difference between EP and EIP groups was also observed.Conclusion： Iron supplement can increase the risk of oxidative stress in spite of improving anemia symptom and lower iron status of exercise rats.Propolis can effectively improve the ability of organism to scavenge free radicals,reduce lipid peroxidation,and enhance antioxidant function.Therefore,propolis can i

关 键 词： 蜂胶 铁补充 运动大鼠 铁状态 抗氧化

领　　域： [农业科学] [农业科学] [轻工技术与工程] [轻工技术与工程]