作　　者： ; ; ; ;

机构地区： 吉林医药学院

出　　处： 《安徽农业科学》 2011年第3期1367-1369,共3页

摘　　要： [目的]提取桑叶多糖并进行含量测定。[方法]采用氢氧化钠提取桑叶中的多糖,从浸提浓度、提取温度、提取时间、料液比4个方面对提取率进行了分析,通过正交试验筛选出碱性提取的最佳工艺条件,得出桑叶多糖氢氧化钠提取的最佳条件。用苯酚-硫酸法测定多糖的含量。[结果]单因素试验得出的最佳条件是提取液浓度为0.5 mol/L,提取温度为80℃,料液比为1∶50,提取时间为4 h。正交试验筛选出桑叶中多糖提取的最佳条件是浸提浓度为1.5 mol/L,提取温度为80℃,料液比为1∶50,提取时间为4 h,多糖含量为2.55%。[结论]提取时间对桑叶多糖的提取有比较大的影响,在单因素试验的基础上通过正交试验得出最佳配比,使得提取更加充分。 [Objective] To extract polysaccharide from mulberry leaf and determine its content.[Method] Polysaccharide was extracted by sodium hydroxide,analysis was made according to the four factors： concentration of NaOH,temperature,time and ratio of solid-liquid,optimum technology condition was selected by orthogonal experiment and optimum condition of extraction of polysaccharide from mulberry leaf by NaOH was found out,the content of polysaccharide was determined by phenol-vitriol.[Result] According to the single element experiment the best concentration of NaOH was 1.5 mol/L,temperature was 80 ℃,time was 4h and the ratio of solid-liquid was 1：50.According to the orthogonal experiment,the best condition was： NaOH was 0.5 mol/L,temperature was 80 ℃,time was 4h and the ratio of solid-liquid was 1：50,the content of polysaccharide was 2.55﹪.[Conclusion] The time of extraction made great difference on extraction of polysaccharide,best ratio could be got by orthogonal experiment on the base of single element to get more sufficient extraction.

关 键 词： 桑叶 多糖 提取 苯酚 硫酸法

领　　域： [农业科学] [农业科学]