作　　者： ; ; ; ; ; ; ;

机构地区： 河北农业大学动物科技学院

出　　处： 《中国兽医科学》 2011年第1期19-24,共6页

摘　　要： 为研究树突状细胞加工和呈递灭活口蹄疫病毒(FMDV)抗原并活化CD8+T细胞的途径,用灭活FMDV负载经抑制剂预处理的小鼠单核细胞源树突状细胞(MoDCs),与CD8+T细胞共培养,对照为负载FMDV的正常MoDCs与CD8+T细胞。收集上清液,检测γ干扰素(IFN-γ)的含量。结果显示,试验组CD8+T细胞在共培养的第9小时分泌大量IFN-γ,然后逐渐下降。至第24小时,CD8+T细胞分泌IFN-γ的量降至最低,随后逐渐升高,在第48小时,CD8+T细胞分泌IFN-γ的量升至最高。对照组CD8+T细胞在共培养的第9小时的IFN-γ分泌量显著低于试验组(P<0.01);随后逐渐升高,并在共培养的第24小时达到高峰,而后逐步下降,至第48小时,CD8+T细胞分泌IFN-γ的量降到最低,显著低于试验组(P<0.01)。结果表明,MoDCs不仅以蛋白酶体依赖途径加工灭活FMDV抗原,而且还可通过蛋白酶体非依赖途径加工灭活FMDV抗原,并通过交叉呈递途径活化CD8+T细胞。 To study the activating pathways of CD8＋T cells by dendritic cells（DCs） pulsed with the inactivated foot-and-mouth disease virus（FMDV）,monocyte-derived dendritic cells（MoDCs） treated with inhibitor were pulsed with the inactivated FMDV and co-cultured with CD8＋T cells.FMDV-pulsed MoDCs co-cultured with CD8＋T cells served as control.The levels of IFN-γ in supernatant were determined at indicated time points with indirect ELISA.CD8＋T cells co-cultured with MoDCs pulsed with the inactivated FMDV released great amount of IFN-γ at 9th hour post-co-cultured and declined gradually,reached the lowest level at 24 h.At 48th hour post-co-cultured,CD8＋T cells recovered their vigorous release of IFN-γ with inactivated FMDV-pulsed MoDCs＇ stimulation.While the release of IFN-γ from control CD8＋T cells was significantly lower at 9th hour（P0.01）,and the lowest level of IFN-γ was detected at 48th hour（P0.01）.Remarkably,the control CD8＋T cells released the highest level of IFN-γ at 24th hour post-co-cultured.These results indicated that MoDCs process inactivated FMDV antigens either in proteasome-independent pathway or in proteasome-dependent pathway and cross-present antigen to CD8＋ T cells,leading to the release of IFN-γ.

关 键 词： 口蹄疫病毒 树突状细胞 抗原呈递 蛋白酶体

领　　域： [农业科学] [农业科学] [农业科学]