作　　者： ; ; ; ; ; ; ;

机构地区： 佛山科学技术学院医学院

出　　处： 《南方医科大学学报》 2011年第2期304-307,共4页

摘　　要： 目的分离纯化幽门螺杆菌(Hp)的野生型毒性噬菌体,并采用体外模拟试验,探讨借口腔Hp保护噬菌体突破胃液屏障、释放子代噬菌体、侵染并治疗胃肠Hp感染的方法。方法以烛缸法培养Hp,用单层平板和双层平板实验分离纯化噬菌体,采用体外模拟胃液环境处理对照组噬菌体和Hp保护的实验组噬菌体,以双层平板实验检测处理后噬菌体的杀菌效果。结果经过模拟胃液处理后,噬菌体溶菌活性随处理时间延长而降低,处理1.5h的实验组依然可形成噬斑,对照组不形成噬斑。结论口腔Hp可以帮助噬菌体抵抗胃液。 Objective To isolate the wild-type virulent phage of Helicobacter pylori（Hp） and simulate the treatments in vitro to investigate the methods for oral Hp-assited penetration of the phage through the gastric barrier and offspring phage release for infection and treatment of gastrointestinal Hp.Methods The Hp strain was cultured with the candle cylinder method and the virulent phage was isolated by single plate or double plate experiment.A simulated gastric juice was applied and the bactericidal effect of the phage was tested with double flats experiment.Results After a 1.5-h treatment in simulated gastric juice,the orally derived Hp-borne phage was still capable of forming plaques while the control phage was not.Conclusion The oral Hp can help the phage resist the gastric juice and then infect the gastrointestinal Hp.

关 键 词： 幽门螺杆菌 毒性噬菌体 生物治疗

领　　域： [生物学]