作　　者： ; ; ; (颜彤）; (欧阳炜）; (刘雁彬）; (努尔亚）;

机构地区： 西北农林科技大学

出　　处： 《辣椒杂志》 2010年第4期29-32,共4页

摘　　要： 以新椒系列辣椒杂交种父母本和一代杂种为材料,采用液氮-SDS法提取辣椒基因组DNA,研究PCR反应的主要影响因子,建立适合辣椒杂交种纯度RAPD分析的PCR反应体系,从120个随机引物中筛选出1个能鉴定新椒10号杂种纯度的引物S1220,从7个随机引物中筛选出1个能鉴定新椒3号杂种纯度的引物S1213。 Some F1 hybrids in Xinjiao hot pepper series and their parents were used as experimental materials in this study.The genomic DNA was extracted with liquid N2-SDS method and the PCR system that was suitable for RAPD analysis of these materials was established.The results showed that primer S1220 could test the purity of F1 hybrid A effectively and S1213 could test the purity of F1 hybrid B effectively.

关 键 词： 辣椒 杂交种 分析 纯度鉴定

领　　域： [农业科学]