作　　者： ; ;

机构地区： 云南农业大学动物科学技术学院

出　　处： 《动物医学进展》 1999年第4期31-34,共4页

摘　　要： 用１，６－二苯基－１，３，５－己三烯（１，６－ｄｉｐｈｅｎｙｌ－１，３，５－ｈｅｘａｔｒｉｅｎｅ， ＤＰＨ， Ｓｉｇ－ｍ ａ Ｃｈｅｍ ｉｃａｌＣｏ．）分子荧光偏振技术研究了内毒素（ＥＴ）诱导的山羊肝线粒体膜（ＭｉＭ）损伤过程中膜脂的流动性（Ｐ），膜脂区微粘度（η）及脂双层分子排列有序性系数（Ａ）的变化及６５４－２（山莨菪碱）的影响。结果表明，ＥＴ处理组（Ⅱ组），肝ＭｉＭＰ、η和Ａ 参均高于对照组（Ⅰ组）和６５４－２处理组（Ⅲ组）（Ｐ＜ ０．０５，Ｐ＜ ０．０１），Ⅲ组Ｐ、η和Ａ 参数在５ ｈ 高于Ⅰ组（Ｐ＜ ０．０５），在１２ ｈ 高于Ⅰ组但无显著性差异（Ｐ＞０．０５）。该结果揭示，ＥＴ可使肝ＭｉＭ 荧光偏振度、微粘度和脂双层分子排列有序性增加，膜流动性降低，而６５４－２ 能有效地改善ＥＴ诱导的这些损伤变化，支持了６５４－２具有维护膜结构和功能的生理作用的理论。 The changes of hepatic mitochondria membrane fluidity and effects of anisodamine were studied with the fluorescence polarization technique, in which 1,6 diphenyl 1,3,5 hexatriene was used as a fluorescence probe, after administration of endotoxin in goats. It was found that after administration of endotoxin, E. coli endotoxin treatment group(group Ⅱ) led to a marked increase of mitochondria membrane fluorescence polarization(P). Microviscocity() and fluorescence anisotropy(A) parameters as compared to the normal control(group Ⅰ) and E. coli endotoxin and anisodamine coexposure group(group Ⅲ)(P<0.05,P<0.01); Three parameters in group Ⅲ were higher than those in group Ⅰ at 5 h (P <0.05) and 12 h(P>0.05). The findings suggested that the hepatic mitochondria membrane changed from a relative fluid to a relative solid after administration of endotoxin, whereas anisodamine had effects of increasing membrane fluidity on liver mitochondria membrane of goats in vivo.

关 键 词： 内毒素 山莨菪碱 膜流动性 线粒体 山羊

领　　域： [农业科学] [农业科学] [农业科学]