作　　者： ; ; ; ; ; ;

机构地区： 深圳职业技术学院应用化学与生物技术学院

出　　处： 《食品研究与开发》 2011年第1期27-30,共4页

摘　　要： 采用高温蒸煮结合纤维素酶酶解的方法,改性豆渣不溶性膳食纤维,制备水溶性膳食纤维。以水溶性膳食纤维的得率为考察指标,分别对高温蒸煮改性、高温蒸煮结合纤维素酶酶解改性工艺进行研究,并应用正交试验设计法优化高温蒸煮结合纤维素酶酶解改性工艺。结果显示,在优化的高温蒸煮结合纤维素酶酶解改性的反应条件下,即固液比为1:20 g/mL、酶解温度为45℃、酶底比为0.3%、酶解时间为4 h、pH值为5.0时,改性后水溶性膳食纤维的得率为31.89%,明显高于单独采用高温蒸煮法(130℃、60 min)改性的水溶性膳食纤维得率(10.85%)。 Soluble dietary fiber（SDF） was prepared from soybean residue insoluble dietary fiber（IDF） by technology of steam-cooking combining with enzymatic hydrolysis.Yield of SDF from IDF was taken as the factor to indicate the technology efficiency.The process technologies of steam-cooking modification and steam-cooking combining with enzymatic hydrolysis modification were studied respectively,and the latter was further optimized by orthogonal design test.The results demonstrated that under the optimized modification condition,i.e.concentration of soybean residue 1：20 g/mL,enzymatic hydrolysis temperature 45 ℃,ratio of cellulase to substrate 0.3 %,hydrolysis time 4 h and pH 5.0,the yield of SDF by steam-cooking combining with enzymatic hydrolysis modification is up to 31.89 %,and it＇s much higher than the yield of SDF only by steam-cooking process（130 ℃,60 min） which was 10.85 %.

关 键 词： 水溶性膳食纤维 不溶性膳食纤维 高温蒸煮 酶解 豆渣

领　　域： [轻工技术与工程] [轻工技术与工程]