作　　者： ; ;

机构地区： 暨南大学生命科学技术学院生物工程学系

出　　处： 《暨南大学学报（自然科学与医学版）》 1999年第5期92-96,共5页

摘　　要： 为探讨光下绿色细胞线粒体氧化磷酸化的调控机理，我们研究了 Ａ Ｔ Ｐ／ Ａ Ｄ Ｐ、 Ｇ Ａ Ｐ（３ － 磷酸甘油醛） 、 Ｄ Ｈ Ａ Ｐ（ 磷酸二羟丙酮） 和 Ｃａ２ ＋ 对豌豆（ Ｐｉｓｕｍ ｓａｔｉｖｕｍ Ｌ．） 叶片分离线粒体氧化磷酸化的影响结果表明：介质中 Ａ Ｔ Ｐ／ Ａ Ｄ Ｐ 在１２ 以下时对线粒体呼吸影响不大，高达１２８ 时才能抑制１８５ ％ ，线粒体外 Ａ Ｔ Ｐ／ Ａ Ｄ Ｐ 可能不是光下绿色细胞线粒体偶联呼吸的抑制因子 Ｇ Ａ Ｐ（４０ ｍｍｏｌ· Ｌ－ １） 和 Ｄ Ｈ Ａ Ｐ（４０ ｍ ｍｏｌ· Ｌ－ １） 抑制线粒体氧化磷酸化分别为８４５ ％ 和７２５ ％ ； Ｃａ２ ＋ （０５ ｍ ｍｏｌ· Ｌ－ １）促进线粒体氧化磷酸化，增至１６８８ ％ ；磷酸丙糖（ Ｇ Ａ Ｐ 和 Ｄ Ｈ Ａ Ｐ） 和 Ｃａ２ ＋ In order to explore the control mechanisms of the mitochondrial oxidative phosphorylation in green cells in light, we have searched the effects of ATP/ADP, GAP (glyceraldehyde phosphate), DHAP (digydroxyacetone phosphate), and Ca 2+ increasing or decreasing the rate of the oxidative phosphorylation of the mitochondria isolated from pea leaves ( Pisum sativum L. ). These indicated that: while the ATP/ADP ratio was below 12 in the medium, it was found to have no any influence on the mitochondrial respiratory rate, even while the ratio was as high as 128, it could only decreased 18.5% of the respiratory oxygen consumption. It is concluded that the ATP/ADP ratio out of mitochondria may not be the factor controlling the mitochondrial coupling respiration of green cells in light. GAP (40 mmol·L -1 ) decreased 84.5% of the mitochondrial oxidative phosphorylation, and DHAP (40 mmol·L -1 ) decreased 72.5%; while Ca 2+ ( 0.5 mmol·L -1 ) increased the mitochondrial oxidative phosphorylation and brought about 168.8%. It is acceptable that the triose phosphate (GAP & DHAP) and Ca 2+ would play important roles in regulating the mitochondrial oxidative phosphorylatory rate of green cells in light.

关 键 词： 线粒体 氧化磷酸化 磷酸丙糖 钙离子 豌豆 光照

领　　域： [生物学]