作　　者： ; ; ; ; ; ; ; (梁敏）;

机构地区： 暨南大学医学院

出　　处： 《畜牧兽医学报》 2011年第1期18-24,共7页

摘　　要： 本研究旨在分析GHRL和GHSR基因部分SNP和单倍型与鸭生长和屠体性状的关联性。试验以三水白鸭为材料,利用PCR-RFLP方法检测GHRL基因C-729T、T+985C和GHSR基因T404C、G3427A共4个SNPs位点的多态性,构建单倍型,进行方差分析。结果表明,C-792T与21、28、35和49日龄体质量、全净膛质量、胸腺质量显著相关(P<0.05),CT基因型杂合子优势明显;T404C与腿肌和脾脏质量显著相关(P<0.05),TT基因型个体均值显著高于CC和TC型个体;G3427A与35日龄体质量、49日龄体质量、屠体质量、半净膛质量、全净膛质量和翅质量显著相关(P<0.05),AA基因型是较为优势的基因型;T+985C与各生长、屠体性状都不相关(P>0.05)。所构建的单倍型与单个SNP的分析结果较为一致,表明这3个SNPs是鸭生长和屠体性状的重要分子标记。 The aim of this study was to analyze the association of GHRL and GHSR genes polymorphisms with duck growth and carcass traits.In this study,two SNPs of GHRL gene（C-729T and C＋985T） and two SNPs of GHSR gene（T404C and G3427A） were genotyped by PCR-RFLP in a random Sanshui White Duck population to investigate the associations of SNP and their haplotypes with growth and carcass traits.Result showed that C-729T was significantly associated with body weight（BW） at 21 days（BW21）,BW28,BW35,BW49,eviscerated weight and Thymus gland weight（P0.05）.Meanwhile,the individuals with CT genotype had better duck body weight gain.T404C was significantly associated with leg muscle weight and spleen weight（P0.05）.And in these traits,the Least-square means of individuals with TT genotype was higher than others.G3427A was significantly associated with BW35,BW49,dressed weight,eviscerated weight with giblet,eviscerated weight and wing weight（P0.05）.And individuals with AA genotype had the highest value of all traits.Otherwise,no significant association of C985T with any growth and carcass traits was found.Association analysis of haplotypes indicated similar results with that of SNP.It was suggested that C-729T of duck GHRL gene and T404C and G3427A of duck GHSR gene were important molecular markers for duck growth and carcass traits.

关 键 词： 鸭 生长性状 屠体性状 关联分析

领　　域： [农业科学] [农业科学] [生物学]