作　　者： ; ;

机构地区： 中山大学

出　　处： 《现代食品科技》 2011年第1期22-25,共4页

摘　　要： 本文探讨了黄斑明目胶囊(含桑椹、菊花、酵母、胡萝卜素、党参、白术、苦茯苓、薏苡仁、丹参,中山眼科中心制)对MNU诱导的大鼠视网膜光感受器细胞变性动物模型的防护作用。将鼠龄为43 d的♀性SD大鼠33只随机分为3组。黄斑明目胶囊组(n=10)以黄斑明目胶囊溶解后灌胃,正常对照组(n=10)和模型对照组(n=10)以等量蒸馏水灌胃,bid,连续7 d。给药7 d后,黄斑明目胶囊组和模型对照组大鼠予MNU 40 mg.kg-1,ip;正常对照组注射等量生理盐水。观察MNU处理后12、24、48、72 h各组大鼠视网膜电图a波及b波的变化,并于MNU作用后7d处死大鼠,取眼球测定视网膜全层及外核层厚度。结果表明正常对照组大鼠不同时间点视网膜电图(ERG)a波及b波的振幅差异无统计学意义(P>0.05);模型对照组在MNU处理后ERG a波及b波的振幅均呈持续性、进行性下降,黄斑明目胶囊可明显抑制ERG a波及b波振幅的降低(P<0.05)。眼病理结果,黄斑明目胶囊组与模型对照组对照组比较,MNU诱导的大鼠视网膜光感受器细胞损伤明显减轻(P<0.05)。 To approach the protective effects of Huangbanmingmu capsule on retinal photoreceptor damage of rats induced by N-Methyl-N-nitrosourea（MNU）,43-day-old female SD rats were randomly divided into control group,model control group and Huangbanmingmu Capsule group.Huangbanmingmu capsule was intragastricly administrated into rats of Capsule group twice one day for continuous 7 days.For Control group and model group,equal water was used.SD rats in 7 days after treatment received a single injection of 40 mg·kg-1 MNU in model group and Huangbanmingmu Capsule group,ip;while control group was given equal normal saline.Change of a wave and b wave of electroretinogram（ERG） were observed in 12,24,48,72 hours after MNU injection.The rats were killed in 7 days after MNU treatment.Retinal full-thickness and outer nuclear layer thickness were determined.Results showed that the difference of the average amplitude of ERG a-wave and b-wave was not significant at different time in control group（P0.05）.And the average amplitude of ERG a-wave and b-wave gradually decreased after MNU treatment in model group.Huangbanmingmu capsule could evidently inhibit lose of a-wave and b-wave,P0.05.Ocular pathology showed that the retinal photoreceptor damage of rats induced by MNU was obviously relieved in Huangbanmingmu Capsule group,compared with model control group（P0.05）.

关 键 词： 黄斑明目胶囊 光感受器细胞 甲基 亚硝脲 视网膜变性

领　　域： [农业科学] [农业科学] [农业科学]