作　　者： ; ; ; ; ;

机构地区： 中国农业科学院北京畜牧兽医研究所

出　　处： 《中国畜牧兽医》 2011年第1期122-125,共4页

摘　　要： 本研究以藏猪、鄂西黑猪、大白猪、莱芜黑猪、北京黑猪、野猪、荣昌猪、八眉猪和里岔黑猪等国内外9个品种16头猪为研究对象,应用Primer Premier 5.0软件设计引物对心脏脂肪酸结合蛋白(heart fatty acid binding protein,H-FABP)基因第2内含子进行扩增测序后拼接,获得了长2078 bp的第2内含子全序列,发现了14个位点的SNPs;对所有突变进行Hudson-Kreitman-Aguade测验等遗传分布分析结果发现,西南型、高原型猪核酸分布值(π)、Tajima’s D测验值(DT)、Fu和Li’s D测验值(DFu and Li)分别为0.18%、-0.52和-0.30,华北型猪π、DT和DFu and Li分别为0.25%、0.76和0.72,培育和国外品种猪π值为0.29%;对所有类型的猪进行分析,其π、DT和DFu and Li分别为0.25%-、0.60和-0.17,在不同的猪种中各个位点的SNPs变异很大,对其进行构建进化树等一系列分析,得到相似结论,并最终推断出可能会影响肌内脂肪(intra muscular fat,IMF)含量的突变位点,为研究H-FABP与IMF的关系提供了理论基础。 DNA resequencing of the H-FABP gene intron 2 in 16 pigs from 9 breeds which were Zang pig,Exi pig,Large White pig,Laiwu Black pig,Beijing Black pig,Wild pig,Rongchang pig,Bamei pig and Licha Black pig respectively revealed 14 polymorphisms in 2078 bp.Hudson-Kreitman-Aguade＇s results showed that a nucleotide diversity（π） of south west type pigs,north type pigs,deloped and imported breeds and all breeds were 0.18%,0.25%,0.29%,0.25%,respectively;the Tajima＇s D value of south west type pigs,north type pigs and all breeds were-0.52,0.76 and-0.60,respectively;the Fu and Li＇s D value of south west type pigs,north type pigs and all breeds were-0.30,0.72 and-0.17,respectively.This results indicated that the SNPs of H-FABP intron 2 were very polymorphic in different breeds,and also,the Neighbour-Joining trees of their haplotypes did not show a geographical arrangement for any of the genes,we inferred some loci which may infect intra muscular fat（IMF）.

关 键 词： 猪 基因 多态性 肌内脂肪 候选基因

领　　域： [生物学]