作　　者： ; ; ;

机构地区： 仲恺农业工程学院生命科学学院

出　　处： 《仲恺农业工程学院学报》 2010年第4期61-63,67,共4页

摘　　要： 以拮抗酵母菌膜醭毕赤酵母(Pichia membranaefaciens)和汉逊德巴利酵母(Debaryomyces hansenii)为试验材料,探讨了培养温度、培养时间、马铃薯葡萄糖液体培养基(PDB)装液量对其生长的影响以及其体外对柑桔指状青霉菌(Penicillium digitatum)的抑菌效果.结果表明,培养温度、培养时间、PDB装液量对2种拮抗菌生长的影响大小依次为:培养温度>培养时间>PDB装液量;汉逊德巴利酵母的生长速度比膜醭毕赤酵母快;拮抗菌对指状青霉菌的抑菌效果与其浓度有关,随着拮抗菌浓度的增加,其抑菌效果变得更加明显,其中1×106 CFU/mL的拮抗菌对指状青霉菌的抑菌效果最好,并且膜醭毕赤酵母的抑菌效果优于汉逊德巴利酵母. Antagonistic yeast Pichia membranaefaciens and Debaryomyces hansenii from citrus were used to study on effects of culture temperature,culture time,PDB amount on their growth and their antimicrobial effects in vitro.The results showed that effects of three factors on antagonistic yeasts growth were culture temperatureculture timePDB amount.The growth rate of Pichia membranaefaciens was faster than that of Debaryomyces hansenii.Antimicrobial effects became more obvious with the increase of the concentration of antagonistic yeasts,1×106 CFU/mL was best concentration.Pichia membranaefaciens was better than Debaryomyces hansenii in the inhibitory effect.

关 键 词： 柑桔 拮抗菌 指状青霉 拮抗作用

领　　域： [农业科学] [农业科学]