作　　者： ; ; ;

机构地区： 北京师范大学生命科学学院

出　　处： 《中国细胞生物学学报》 2010年第6期902-907,共6页

摘　　要： RNAi和GFP报告基因的转染技术是细胞生物学领域常用的研究手段,我们以GFP作为靶基因,设计了适合在本科生课程中开设的RNA干扰技术实验项目,本教改研究将GFP荧光观察、基因转染技术及RNA干扰技术融合在一个实验中,并且对该实验项目的实例应用做了细致的设计和具体实践,结果表明RNAi技术对细胞中GFP报告基因表达的抑制效果明显。此外本文还阐述了该实验项目在本科生的细胞生物学实验课程中开展的可行性和必要性。这种将传统技术与新技术交叉组合型实验项目的开展对于学生综合科研能力的培养具有良好的效果,也为细胞生物学实验课程的改革提供了新思路。 RNAi and GFP gene transfer technique are commonly used in cell biology research field.With GFP as the target gene,we developed this RNAi experiment project that is suitable for undergraduate students.In this project,we integrated GFP fluorescence observation,gene transfer and RNA interference technique into one project.Also we designed its detailed protocol and did concrete practice for this experiment project.Results showed that RNAi technique could significantly inhibit the expression of GFP gene in cell,so we drew the conclusion that this experiment project had good feasibility and necessity to be carried out in cell biology experiment course for undergraduates.This cross-cutting,integrated experiment projects should have a good effect for students＇ integrated research ability,and also provide a novel idea for cell biology experiment course reform.

关 键 词： 细胞生物学实验

领　　域： [生物学]