作　　者： ; ; ; ; ; ;

机构地区： 龙岩学院生命科学学院

出　　处： 《中国预防兽医学报》 2010年第12期988-990,共3页

摘　　要： 为鉴定猪蛔虫与感染相关的差异表达基因,本实验由猪蛔虫感染期幼虫差异消减cDNA文库中挑选EST序列(28A02)为研究对象,首先,经半定量RT-PCR方法分析该基因在各期虫体的表达情况。结果显示:该基因在猪蛔虫感染期幼虫和肺三期幼虫中均有表达,并且在感染期幼虫表达丰度最高,在其它各期虫体包括雌虫、雄虫、虫卵和第四期幼虫均未检测出该基因的表达。其次,以已知的EST序列为模板,采用RACE技术获得了864 bp的全长cDNA序列,包含一个完整的长约450 bp的开发阅读框。另外,经生物信息学分析显示,该基因编码的氨基酸序列与众多物种的ATP合酶有43%～47%的相似性,推测该基因可能参与编码猪蛔虫的线粒体ATP合酶,这为进一步研究其功能奠定了基础。 In the present study,semi-quantitative RT-PCR was used to examine the expression profiles of an EST sequence（28A02） of Ascaris suum infective larvae at different developmental stages.The full-length sequence of 28A02 gene was obtained using rapid amplification of cDNA ends and the characteristics of predicted protein were analyzed.The results showed that the expression level of the 28A02 gene in the infective larvae was much higher than L3 stage in lung and no expression were detected in eggs,L4 or adults.The full-length cDNA was 864 bp in size,encoding 149 amino acids.Amino acid sequence alignment indicated that the 28A02 shared 43% to 47% identity with ATP synthase of other species.

关 键 词： 猪蛔虫 感染期幼虫 基因 半定量 全长 生物信息学分析

领　　域： [农业科学] [农业科学] [农业科学]