作　　者： ; ; ; ;

机构地区： 兰州城市学院体育学院

出　　处： 《体育科学》 2010年第12期76-81,86,共7页

摘　　要： 目的:探寻过度运动引起的心肌过氧化损伤活性氧产生的途径,实施活性氧产生途径上的干预与营养学上的抗氧化防护,为运动引起的心肌过氧化损伤的抗氧化干预提供方法学上的理论支撑;方法:85只雄性waster大鼠,随机分为安静对照组(C)、单纯过度运动组(E)、过度运动给予DPI干预组(D)、过度运动给予谷氨酰胺干预组(G)、过度运动合并给予DPI与谷氨酰胺干预组(DG),建立过度训练模型后的36h和恢复7天后测定其血浆中的心肌肌钙蛋白(cTnI)、肌酸同工酶(CK-MB)浓度以及心肌组织中的丙二醛(MDA),实时定量PCR测定运动后心肌细胞NADPH氧化酶关键亚基gp91-phox和p22-phox的mRNA的表达;结果:与对照组比较,E组血浆MDA、cTnI、CK-MB均显著升高,恢复7天后显著下降,D、G组则升高幅度低于E组,DG组则变化不明显,但干预组升高的幅度则显著低于单纯训练组。运动后36h的gp91-phox、p22-phox的mRNA的表达也表现出同样的变化特点;结论:过度训练可引起心肌的过氧化损伤,其中,NADPH氧化酶介导产生的活性氧是其过氧损伤的原因之一,NADPH氧化酶的抑制剂与谷氨酰胺合并时使用具有协同的抗氧化防护作用,可有效地防护过度运动引起的心肌过氧化损伤。 Objective：To explore the pathways of reactive oxygen species（ROS）induced by exhaustive exercise that damage myocardial cell and to put anti-oxidant intervention into practice with the combination both the way of generating ROS and the nutrition.Methods：85 male Wistar rats were randomly divided into five groups and established protocol of exhaustive exercise.The five groups is sedentary control group（C）,exhaustive exercise group（E）,exhaustive exercise and DPI intervention group（D）,exhaustive exercise and glutamine intervention group（G）,exhaustive exercise and the combination of DPI and glutamine intervention group（DG）.The concentration ofmalondialdehyde（MDA）,cardiac troponin T（cTnT）,creatine isoenzyme（CK-MB）in rat blood plasma were determined at 36-40 hours after exhaustive exercise and 7 days recovery respectively.The expression of FAS /FASL of mRNA and the NADPH oxidase subunit gp91-phox p22-phox of mRNA in cardiac myocyte were detected with real-time quantitative PCR.Results：Compared with control group C,the concentration of MDA,cTnI,CK-MB in E group were increased significantly after 7 days recovery were decreased significantly and the concentration in group D and group G also increased no significantly and in DG group did not change.The expression of the FAS /FASL,gp91-phox,p22-Phox of mRNA also showed the same results as the plasma index in all groups at 36h after exercise.Conclusion：The exhaustive training can cause excessive oxidative damage in the heart muscle.The one pathway of ROS generated by NADPH oxidase is the one reasons of the peroxide injury of myocardial cell.So the combination intervention with the inhibitors of NADPH oxidase and glutamine supplement can protect the oxidative injury in myocardial cell induced by exhaustive exercise effectively.

关 键 词： 大鼠 过度运动 氧化酶 活性氧 心肌细胞 过氧化损伤 二联苯碘 谷氨酰胺

领　　域： [文化科学] [文化科学]