作　　者： ; ; ;

机构地区： 浙江工业大学化学工程与材料学院

出　　处： 《环境科学》 2011年第1期225-230,共6页

摘　　要： 从珠海赤潮海水中分离出2株溶藻细菌Y01和Y04,对球形棕囊藻均有显著的溶藻效果.结果表明,溶藻细菌Y01和Y04均为革兰氏阳性菌,对球形棕囊藻的溶解作用时间为6 d.借助显微镜及扫描电镜等观察Y01和Y04溶藻过程,发现2株溶藻细菌的溶藻方式均为直接裂解藻细胞.溶藻细菌Y01和Y04的16S rDNA系列分析表明,溶藻细菌Y01和Y04的PCR扩增产物的长度分别为1 468 bp和1 548 bp(登录号分别为DQ531607和DQ531608).菌株Y01和Y04与多株芽孢杆菌的16S rDNA核苷酸序列的同源性在99.7%以上,均归属于芽孢杆菌属. The algae-lysis bacteria Y01 and Y04 were isolated from seawater, during a red tide survey of the Zhuhai Estuary in southern China. The algae-lysis bacteria YO1 and Y04 were Gram-positive bacteria which can remove P. globosa in 6 days. The lysing process was observed by microscope and SEM, both of them could directly lyse P. globosa. The strains were identified by the sequence analysis of l6S rDNA. The length of the two bacteria＇s sequences were 1 468 bp and 1 548 bp （strain Y01： DQ531607; strain Y04： DQ531608）. The DNA sequence similarity searches showed that share more than 99.7% sequence homology with some strain of Bacillus. The algae-lysing bacteria would provide a possibility to control P. globosa red-tide by microbial agents.

关 键 词： 球形棕囊藻 溶藻细菌 溶藻特征 有害赤潮 芽孢杆菌

领　　域： [环境科学与工程]