作　　者： ; ; ;

机构地区： 华南理工大学轻工与食品学院

出　　处： 《食品与发酵工业》 2010年第11期93-97,共5页

摘　　要： 探讨了单酶及双酶分步酶解鸭蛋清蛋白制备多肽的工艺和水解效果。在7种不同来源的蛋白酶中,蛋清水解专用蛋白酶、碱性蛋白酶Alcalase AF 2.4L和胰蛋白酶均显示出了较好的酶解效果,其中以碱性蛋白酶Alcalase AF 2.4L酶解效果最佳,酶解的最佳工艺条件为:底物浓度为50g/L,反应时间4 h,加酶量8.0×104 U/g,pH值8.3、60℃,最大水解度为10.0%;而以蛋清水解专用蛋白酶和胰蛋白酶为催化剂时最大水解度分别为8.8%和7.2%。根据单酶酶解结果探讨了双酶分步酶解工艺,确定了以"蛋清水解专用蛋白酶-碱性蛋白酶Al-calase AF 2.4L"和"碱性蛋白酶Alcalase AF 2.4L-胰蛋白酶"的双酶组合较好,鸭蛋清蛋白在酶解8h后水解度可达到26.2%和26.6%,明显优于单酶水解效果。 The hydrolysis of duck egg-white protein was investigated for preparation of peptides using single and binary-enzymes. Seven kinds of proteases were tested for the hydrolysis of egg-white protein, among which Alcalase AF 2.4L showed the highest catalytic capability. Besides, the egg-white protein-specific protease （SEEP） and trypsin also exhibited high activities in the hydrolysis reaction. By single-factor and orthogonal tests, the optimal conditions for egg white protein hydrolysis were determined as follows： substrate concentration 50 g/L, reaction time 4 hours, en-zyme dosage 8.0 × 10^4 U/g, pH value 8.3, and reaction temperature 60℃ , under which the DH values of hydrolysis reactions catalyzed by Alcalase AF 2.4L, SEEP and trypsin were 10.0 % , 8.8 % and 7.2 %, respectively. According to the single-enzyme catalyzed hydrolysis, the most suitable binary combinations of proteases were determined as SEEP- Alcalase AF 2.4L and Alcalase AF 2.4L-trypsin. It was found that the degrees of hydrolysis were significantly improved by using SEEP-Alcalase AF 2.4L and Alcalase AF 2.4L-trypsin, being of 26.2 % and 26.6 % , respectively.

关 键 词： 鸭蛋清蛋白 多肽 双酶 水解度

领　　域： [轻工技术与工程]