作　　者： ; ; ; ;

机构地区： 浙江农林大学亚热带森林培育国家重点实验室

出　　处： 《浙江林业科技》 2010年第4期50-52,共3页

摘　　要： 采用改良CATB法对采自云南大青山、平果等9个天然分布区的150个西南桦(Betula alnoides)样品进行DNA提取,获得了较高质量的DNA,然后利用AFLP分子标记技术,建立了适用于西南桦的AFLP反应体系;选择5对AFLP引物,对150个西南桦样品进行扩增,共检测出158个位点,其中多态性位点115个,占72.8%,每对引物获得20～55个位点,多态性比例为50.0%～84.0%。通过对西南桦的遗传多样性分析,结果表明:在种间个体水平上,多态性位点百分率为76.58%～93.04%,平均为83.8%;在群居水平上,多态性位点百分率为72.80%。可见西南桦天然居群内的变异大于居群间的变异;由Nei’s遗传距离分析得出,西南桦大部分居群间还是存在一定的遗传距离,其中最大在东兰和靖南之间,为0.020 1。 Extraction of DNA was conducted by improved CATB from 150 samples of Betula alnoides at 9 natural communities in Daqingshan,Pingguo,etc.Yunnan province.And AFLP reaction system was established by AFLP molecular marker.Five EcoRI/MseI primer combinations were selected and amplified 158 clear bands,among them 115 bands of polymorphic,taking 72.8%.Each primer enzyme mix amplified 20-55 bands,50.0%-84.0% of them polymorphic bands.Analysis on genetic diversity showed that interspecific percentage of polymorphic loci was 76.58%-93.04%,average 84.05%,community percentage of polymorphic loci was 72.80%,indicating that more variation in community than that among communities.Analysis of Nei’s showed that there is significant genetic distance among most natural communities of B.alnoides.The biggest is 0.0201 between Donglan and Jingnan.

关 键 词： 西南桦 遗传多样性 分子标记 多态性

领　　域： [农业科学] [农业科学]