作　　者： ; ; ; ; ;

机构地区： 韶关大学英东生物工程学院农业工程系

出　　处： 《化学进展》 2010年第12期2408-2419,共12页

摘　　要： 双光子吸收是指在强光激发下,介质分子同时吸收两个光子,从基态跃迁到两倍光子能量的激发态的过程。荧光显微成像是研究活体生物的重要工具,而最通常的细胞成像方法则是使用单光子激发荧光团的单光子显微成像。近红外光源激发的双光子荧光探针克服了单光子荧光探针的光漂白与光致毒而更适于生物检测与成像,为生命科学研究提供了更为锐利的工具。双光子荧光探针的作用机理包括分子内电荷迁移(ICT)、荧光共振能量迁移(FRET)、光诱导电子迁移(PET)与基团转换(GC)4种方式。该文综述了双光子阳离子探针(Mg2+,Ca2+,Pb2+,Hg2+,Ag+,Fe3+,Zn2+,Na+,Cr3+)、双光子阴离子探针(F-)、pH探针、双光子葡萄糖示踪器、双光子脂筏探针、双光子巯基探针、双光子半胱氨酸探针和双光子生物标记探针,以及双光子荧光探针在生物成像方面的应用,展望了双光子荧光探针的发展趋势与应用前景。 Two-photon absorption is a process in which a molecule in medium simultaneously absorbs two photons,and performs transition from ground state to excited state with the energy of two photons.Optical imaging with fluorescence microscopy is a vital tool in the study of living cells and tissues,whereas the most common method for cell imaging,one-photon microscopy（OPM）,uses a single photon of higher energy to excite the fluorophore.By using NIR（near infrared） photons as excitation source,two-photon fluorescence sensors which can avoid photodamage and photobleaching induced by one-photon fluorescence sensors are quite suitable for bioassay and bioimaging,and become powerful tools for the study on life science.The recognition mechanisms of two-photon fluorescence probes are comprised of the intramolecular charge transfer（ICT）,the fluorescence resonance energy transfer（RET）,the photoinduced electron transfer（PET） and the group conversion（GC）.Two-photon probes for cation ions（Mg^2 ＋,Ca^2 ＋,Pb^2 ＋,Hg^2 ＋,Ag^ ＋,Fe^3 ＋,Zn^2 ＋,Na ^＋,Cr^3 ＋）,two-photon anion ion probes（F-）,two-photon pH probes,two-photon glucose tracers,two-photon lipid raft probes,two-photon sulfhydryl group probes,two-photon cysteine probes and two-photon biolabelling probes,and the applications of two-photon fluorescence probes in bioimaging are reviewed.In particular,the research progress,developing trends and prospects in the future are also discussed.

关 键 词： 双光子荧光探针 双光子生物成像 双光子吸收截面 双光子激发荧光

领　　域： [理学] [理学] [理学] [理学]