作　　者： ; ; ; ; ;

机构地区： 广东海洋大学食品科技学院

出　　处： 《水产学报》 2010年第11期1783-1788,共6页

摘　　要： 为了研究麻痹性贝类毒素(paralytic shellfish poisoning toxins,PSP)的来源和检测,对实验室分离培养的产麻痹性贝类毒素的链状亚历山大藻进行了研究。首先通过接种对数生长期藻细胞,培养8d内就能达到最高细胞密度(2.4×104cells/mL)左右,再以0.05mol/L的醋酸超声波破碎藻细胞提取粗毒素,结合小白鼠生物检测法,经高效液相色谱分析,本株藻主要含有C1/2、GTX4、GTX5和NEO,浓度分别为GTX5(0.8275)>GTX4(0.3392)>C2(0.2526)>NEO(0.1266)>C1(0.0455)(单位:μmol/L),毒素粗提液经过Sephadex-G15凝胶层析柱处理,用小白鼠生物法和荧光分光光度法联合检测定位,收集到纯度较高的PSP,本论文工作为将来贝类毒素的研究提供资料。 In order to accumulate the basic data and the raw material for future research,paralytic shellfish poisoning（PSP） of Alexandrium catenella cultured in the laboratory was studied.Firstly,in the logarithmic phase the algae cells were inoculated,the cell growth rate kept higher in initial 8 days.After it attained the highest density of cell（2.4 × 104 cells/mL） ,the algae were collected by filtration,the crude toxin was extracted by 0.05 mol/L acetic acid.Combined with the mice bioassay,the crude toxin was identified.The result showed that the major toxic component from A.catenella were C1 /2,GTX4,GTX5 and NEO by high performance liquid chromatography（HPLC） and the concentrations of them were about 0.045 50,0.252 6,0.339 2,0.827 5,0.126 6 μmol/L respectively.The crude toxin was purified via Sephadex-G15 column chromatography and detected by fluorescence spectroscopy and the mice bioassay,and the result showed that the impurities can be effectively excluded by Sephadex-G15 column chromatography.

关 键 词： 链状亚历山大藻 麻痹性贝类毒素 高效液相色谱 荧光分光光度法 小白鼠生物检测

领　　域： [生物学] [农业科学]