作　　者： ; ; ; ; ; ;

机构地区： 华南师范大学生命科学学院

出　　处： 《园艺学报》 2010年第11期1843-1850,共8页

摘　　要： 抗病转录因子Pti4是从番茄中分离的一种能够调控番茄抗病基因Pto转录的蛋白质。通过根癌农杆菌介导法将抗病转录因子基因Pti4导入花椰菜无菌苗下胚轴,同时对转基因植株进行了PCR、PCR-Southern和Southern blot分子检测以及细菌性黑腐病和软腐病病菌接种试验。结果表明,在建立再生系统过程中外植体的最佳苗龄为5~7d,培养基中激素组合为NAA0.2mg·L-1、6-BA2.0mg·L-1时不定芽的诱导和分化效果最好。获得的35株阳性植株经分子检测证明抗病转录因子基因Pti4已被整合到花椰菜的基因组中。对其中26株接种病菌发现Pti4转化植株对细菌病害具有一定的抗性。 The disease resistance-related transcription factor Pti4 is a kind of protein isolated from tomato which can regulate the transcription of disease-resistance gene Pto.In this paper,Pti4 was transferred into the hypocotyls of cauliflower（Brassica oleracea var.botrytis L.） mediated by Agrobacterium tumefaciens,and the transgenic plants were detected by PCR,PCR-Southern blot,Southern blot and the inoculation assay of bacterial soft rot and black rot.The results indicated that the optimal conditions for induction and differentiation of adventitious buds were 5-7 day-old seedling,coupled with MS medium supplemented with 0.2 mg · L-1 NAA and 2.0 mg · L-1 6-BA.Furthermore,35 positive transgenic plants were obtained and confirmed by molecular detection,which approved that the disease resistance-related transcription factor gene Pti4 was integrated into cauliflower genome.Among them,26 transgenic plants were more resistant than non-transgenic ones in disease-resistance assay.

关 键 词： 花椰菜 抗病转录因子 基因 转化

领　　域： [农业科学] [农业科学]