作　　者： ; ; ; ; ; ;

机构地区： 中国水产科学研究院南海水产研究所

出　　处： 《食品科学》 2010年第20期173-177,共5页

摘　　要： 采用酸水解法对琼胶进行降解并结合苯酚-硫酸法测定琼胶低聚糖的含量。在单因素试验的基础上,利用响应曲面法优化水解工艺,建立盐酸浓度(X1)、水解时间(X2)和固液比(X3)与水解率(Y)之间的数学模型:Y=81.5933+0.7175X1+1.8938X2+2.7313X3-3.1567X12-3.3042X22-3.3092X32-0.6625X1X2+0.0475X1X3-0.8000X2X3,确定琼胶水解的最佳工艺条件,即盐酸浓度0.105mol/L、水解时间96min、固液比4.5:100(g/mL),在此最佳工艺条件下进行水解,琼胶水解率为81.43%。 Agar was subjected to acid hydrolysis and the content of agar oligosaccharides was determined by hydrochloric acid and phenol-sulfuric acid method. On the basis of single factor experiments,the hydrolysis procedure was optimized using response surface methodology,and a mathematical regression model （Y= 81.5933＋0.7175X1＋1.8938X2＋2.7313X3-3.1567X12-3.3042X22-3.3092X32-0.6625X1X2＋0.0475X1X3-0.8000X2X3） was established for predicting the yield of agar oligosaccharides （Y） at different levels of hydrochloric acid concentration （X1）,hydrolysis time （X2）,and solid/liquid ratio （X3）. The optimal hydrolysis conditions were determined to be hydrochloric acid concentration of 0.105 mol/L,solid/liquid ratio of 4.5：100 （g/mL） and hydrolysis time of 96 min. The yield of agar oligosaccharides was up to 81.43% under the optimal hydrolysis conditions,which was in good agreement with the predicted value.

关 键 词： 琼胶 酸水解 响应曲面法

领　　域： [轻工技术与工程] [轻工技术与工程]