机构地区: 河北农业大学
出 处: 《食品科技》 2010年第11期311-315,共5页
摘 要: 针对转基因大豆的特异基因CP4-EPSPS(GenBank accession numberAY5966948)设计引物,用LAMP引物在线设计软件设计引物。对反应温度、反应时间、镁离子浓度、Bst DNA聚合酶浓度等扩增条件进行优化,建立了LAMP反应体系,检出限为0.01%。 The specific LAMP primers of Genetically Modified Soybean were designed on the basis of the published sequence of strain CP4-EPSPS(GenBank accession number AY5966948) with the LAMP primer design support software program,using primer explorer software.The reaction conditions wereoptimized including temperature,time,the concentration of Mg2+,concentration of Bst DNA polymerase etc,the detection limits of LAMP were 0.01%.