作　　者： ; ; ; ;

机构地区： 浙江大学生物系统工程与食品科学学院

出　　处： 《中国食品学报》 2010年第5期131-136,共6页

摘　　要： 基于絮凝素FLO1基因片段为锚定序列,构建以诱导型GAL1启动子介导的表达载体YEP-GMPFAK,并实现以半乳糖为诱导剂,β-1,3-1,4-葡聚糖酶在酿酒酵母表面的展示表达。酵母转化子发酵实验表明,在pH6.0、50℃培养条件下,经2%半乳糖诱导24 h,表面展示酶活达到最大值322.83 U/g细胞干重。酶学性质测定表明,展示表达的β-1,3-1,4-葡聚糖酶具有良好的热稳定性。 A yeast surface-display expression vector YEP-GMPFAK mediated by inducible GALl promoter and based on floeeulation （FLO1） gene fragment as an anchor was constructed. When industry Saccharomyces cerevisiae was transformed using this vector and then induced by galactose, β-1,3-1,4-glucanase was expressed on yeast surface. Fermentation experiment showed that the β-1,3-1,4-glucanas activity displayed on yeast surface reached 322.83 U/（g.dry cell weight） at the cultivating condition of 50 ℃, pH 6.0,after adding 2% galactose for 24 h. The deetection results of enzymatic properties showed that the surface-displayed β-1,3-1,4-glucanase also exhibited favorable thermal stability.

关 键 词： 酿酒酵母 诱导 酵母表面展示 葡聚糖酶

领　　域： [轻工技术与工程] [轻工技术与工程]