作　　者： ; ; ; ; ; ;

机构地区： 南方医科大学中医药学院

出　　处： 《中国实验方剂学杂志》 2010年第14期31-34,共4页

摘　　要： 目的:研究甘蔗渣中多糖的最佳纯化工艺。方法:用Savage法、TCA法脱蛋白;用双氧水法、活性炭法脱色;用AB-8,DB301,D101,MG-1大孔树脂纯化甘蔗渣多糖,用苯酚-硫酸法测定多糖的含量。结果:最佳工艺为用Savage法脱蛋白、活性炭脱色、MG-1型大孔吸附树脂纯化(上样量为0.3 g,柱高35 cm、流速10 mL/15 min),经富集后甘蔗渣多糖的含量由51.11%提高到了83.57%。结论:本实验为甘蔗渣多糖的分离纯化条件提供了参考,为甘蔗渣的进一步开发研究提供了依据。 Objective：To explore the optimal purification process of the polysaccharides from sugarcane biogases.Method：To explore the optimum technology,we compared Savage method with TCA method for depro-teinization and hydrogen peroxide solution method with active carbon method for decoloration.And four common macroporous absorption resins were compared for differences in adsorption and desorption performance.Then phe-nol-sulfuric acid method was used to determine the content of the polysaccharides from sugarcane biogases.Result：The optimum purification technological conditions were determined as following：deproteinization with savage meth-od;discoloration with active carbon method;purification with macroporous absorption resins of MG-1（the upper sample weight is 0.3 g;the column high is 35 cm and the flow rate is 10 mL /15 min）.The polysaccharides of sug-arcane biogases improved from 51.11% to 83.57%.Conclusion：This experiment provided a reference for the pur-ification of polysaccharides from sugarcane biogases and provided an evidence for the further development of sugar-cane biogases.

关 键 词： 甘蔗渣多糖 脱蛋白 脱色 大孔吸附树脂 纯化工艺

领　　域： [哲学宗教]